What it does, reaction by reaction
Gluconeogenesis Carbohydrate Metabolism · Cytosol + mitochondrion + ER
Phosphoenolpyruvate + H2O ↔ 2-phosphoglycerate
Converts Phosphoenolpyruvate H2O into 2-Phosphoglycerate
› Notes
Enolase (Mg2+-dependent) catalyzes the reverse of glycolytic dehydration. This reaction is reversible.
Glycolysis Carbohydrate Metabolism · Cytosol
2-Phosphoglycerate → phosphoenolpyruvate + H2O
Converts 2-Phosphoglycerate into Phosphoenolpyruvate H2O
› Notes
Enolase requires Mg2+ (or Mn2+) and reversibly dehydrates 2-phosphoglycerate to form phosphoenolpyruvate (PEP), a compound with high phosphoryl-transfer potential. Fluoride inhibits enolase and is used to limit glycolysis in some blood collection tubes.
Anaerobic Glycolysis / Lactic Acid Fermentation Energy Metabolism & Cellular Respiration · Cytosol
2-Phosphoglycerate ⇌ phosphoenolpyruvate + H2O
Converts 2-Phosphoglycerate into Phosphoenolpyruvate H2O
› Notes
Required cofactors: Mg2+. The reaction is reversible; fluoride inhibits enolase and is used in some blood-collection tubes to limit ex vivo glycolysis.
Showing all 3 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
Europe PMC · fetched just now · sorted by publication date
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1
Unveiling the cognitive fog in lung cancer patients: non-invasive exploration of blood-brain barrier disruption and brain structural changes.
Zhang DF, Ma H, Zhang ZP, Ai J, Cao WT, Li ZH. · 2026-06-18
open access unreviewed -
2
High thrombin activity associated with crohn's disease induces microbiota pathogenicity contributing to mucosal inflammation.
Le Cosquer G, Pannier M, Thevenin J, Guiraud L, David M, Dumas A, Meunier E, Rousset P, Palese S, Giorgio C, … · 2026-06-17
open access unreviewed -
3
A synopsis of competence-regulated virulence in <i>Streptococcus pneumoniae</i>.
Chong SY, Lew SQ, Lau GW. · 2026-05-25
open access unreviewed -
4
Identification of proteins interacting with the N-terminal half of endoribonuclease RNase E in <i>Escherichia coli</i>.
Göpel Y, Durica-Mitic S, Boyko S, Dudys P, Amman F, Schnetz K, Görke B. · 2026-06-02
open access unreviewed -
5
Metabolic memory in the kidney: how lactate and lactylation drive the path from acute injury to chronic disease.
Zhang X, Dai Q, Feng H. · 2026-05-20
open access unreviewed -
6
Wt1 facilitates visceral beige fat formation to combat abdominal obesity.
He LF, Liao ZM, Chen JL, Yang ZC. · 2026-05-13
open access unreviewed -
7
Beyond spherical vesicles: morphological diversity of extracellular vesicles in health and disease.
Velázquez-Cervantes MA, Fierro NA, Lozano-Hernández R, Carrero JC. · 2026-05-04
open access unreviewed -
8
Glucagon-like peptide-1: a critical link between gut microbiota dysbiosis and degenerative musculoskeletal diseases.
Yang W, Hao C, Wang N, Xie J, Zhou B, Yang Z, Zheng A, Wei J, Li C, Xie C, Li H, Lei G, Zeng C. · 2026-04-22
open access unreviewed -
9
Early diagnosis of a case of Heidenhain variant of Creutzfeld-Jakob disease by cerebrospinal fluid real-time quaking-induced conversion test.
Li L, Yan R, Wang J, Guo Y. · 2026-03-27
open access unreviewed -
10
Emerging roles of RNA N5-methylcytosine modification in reproductive physiology and gynecological diseases.
Lv F, Jin Y, Yu S, Yang X, Xia S, Ye X, Zhao Q, Hu Y, Zheng A, Wu Y, Tong J. · 2026-03-04
cited 1× open access unreviewed
External claims. These come from an index outside this database and are not checked against it. Treat them as leads.