What it does, reaction by reaction
Gluconeogenesis Carbohydrate Metabolism · Cytosol + mitochondrion + ER
Phosphoenolpyruvate + H2O ↔ 2-phosphoglycerate
Converts Phosphoenolpyruvate H2O into 2-Phosphoglycerate
› Notes
Enolase (Mg2+-dependent) catalyzes the reverse of glycolytic dehydration. This reaction is reversible.
Glycolysis Carbohydrate Metabolism · Cytosol
2-Phosphoglycerate → phosphoenolpyruvate + H2O
Converts 2-Phosphoglycerate into Phosphoenolpyruvate H2O
› Notes
Enolase requires Mg2+ (or Mn2+) and reversibly dehydrates 2-phosphoglycerate to form phosphoenolpyruvate (PEP), a compound with high phosphoryl-transfer potential. Fluoride inhibits enolase and is used to limit glycolysis in some blood collection tubes.
Anaerobic Glycolysis / Lactic Acid Fermentation Energy Metabolism & Cellular Respiration · Cytosol
2-Phosphoglycerate ⇌ phosphoenolpyruvate + H2O
Converts 2-Phosphoglycerate into Phosphoenolpyruvate H2O
› Notes
Required cofactors: Mg2+. The reaction is reversible; fluoride inhibits enolase and is used in some blood-collection tubes to limit ex vivo glycolysis.
Showing all 3 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
Europe PMC · fetched just now · sorted by publication date
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1
A user-friendly strategy to engineer tailored intermediate strains for overcoming combined type I and type II restriction-modification barriers in <i>Staphylococcus aureus</i>.
Zheng Y, Li C, Huang X, Shou X, Su R, Zhang J, Hu Q, Shang W, Rao X, Zhou R, Jiang XR. · 2026-07-03
open access unreviewed -
2
Metabolic reprogramming in colorectal cancer: Mechanisms and therapeutic insights.
Xiao W, Tan D, Zeng W, Nie X, Wang M. · 2026-01-28
open access unreviewed -
3
ENO1 as a potential tumor prognostic marker promotes the proliferation, invasion, and metastasis of breast cancer cells.
He L, Xu Y, Huang L, He L, Chen Z, Qi Z, Cui Y. · 2026-04-28
open access unreviewed - 4
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5
Nanozyme-catalyzed dual-potential electrochemiluminescence immunosensor for simultaneous detection of CEA and NSE as lung cancer biomarkers.
Cui C, Liu N, Nian Q, Wang X, Wang S, Sun W, Xia Y, Jiang D. · 2026-07-11
unreviewed -
6
Tissue-specific transcriptomic responses and skeletal-muscle ultrastructural observations in Semah mahseer (<i>Tor douronensis</i>) under acute thermal and hypoxic conditions.
Uchuwittayakul A, Phuthong W, Kong C, Rajitdumrong C, Adisornprasert Y, Kumwan B, Meachasompop P, Ely SSP, Va… · 2026-08-22
open access unreviewed -
7
Predictors of cardiac autonomic neuropathy in patients with diabetic peripheral neuropathy.
Abdel Reheem AA, Hassan AA, Abd-Allatif NE, Tahoun MM, Kassab HS. · 2026-08-31
unreviewed -
8
Pathogenicity and virulence of <i>Streptococcus suis</i> serotype 2: Emerging insights and persistent knowledge gaps.
Fang L, Yao Y, Wu J, Ning J. · 2026-07-28
open access unreviewed -
9
Aggrecan and polymeric immunoglobulin receptor in extracellular vesicles of patients with seropositive rheumatoid arthritis.
Castaño-López S, Lopera TJ, Restrepo V, Carbal LF, Ramírez-Mejía JM, Hernández A, Gil D, Díaz JC, Manrique-Mo… · 2026-07-08
open access unreviewed -
10
Decoding osteosarcoma pathogenesis: the pivotal influence of m<sup>6</sup>A modification.
Huang C, Han Z, Su J, Zhang M, Lin Y, He H, Tang Y, Guo P, Wang Y, Chen H. · 2026-07-21
open access unreviewed
External claims. These come from an index outside this database and are not checked against it. Treat them as leads.