What it does, reaction by reaction
Gluconeogenesis Carbohydrate Metabolism · Cytosol + mitochondrion + ER
Phosphoenolpyruvate + H2O ↔ 2-phosphoglycerate
Converts Phosphoenolpyruvate H2O into 2-Phosphoglycerate
› Notes
Enolase (Mg2+-dependent) catalyzes the reverse of glycolytic dehydration. This reaction is reversible.
Glycolysis Carbohydrate Metabolism · Cytosol
2-Phosphoglycerate → phosphoenolpyruvate + H2O
Converts 2-Phosphoglycerate into Phosphoenolpyruvate H2O
› Notes
Enolase requires Mg2+ (or Mn2+) and reversibly dehydrates 2-phosphoglycerate to form phosphoenolpyruvate (PEP), a compound with high phosphoryl-transfer potential. Fluoride inhibits enolase and is used to limit glycolysis in some blood collection tubes.
Anaerobic Glycolysis / Lactic Acid Fermentation Energy Metabolism & Cellular Respiration · Cytosol
2-Phosphoglycerate ⇌ phosphoenolpyruvate + H2O
Converts 2-Phosphoglycerate into Phosphoenolpyruvate H2O
› Notes
Required cofactors: Mg2+. The reaction is reversible; fluoride inhibits enolase and is used in some blood-collection tubes to limit ex vivo glycolysis.
Showing all 3 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
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