CPS-II domain of carbamoyl-phosphate synthetase II (CPS-II)
Cofactors used
What it does, reaction by reaction
Pyrimidine De Novo Synthesis (through to UMP, then CTP/dTMP) Nucleotide Metabolism · Cytosol (one mitochondrial step)
Glutamine + bicarbonate → carbamoyl phosphate
Converts Glutamine HCO3- into Carbamoyl phosphate
› Notes
The CPS-II domain of carbamoyl-phosphate synthetase II (CPS-II) catalyzes glutamine + HCO3− + 2 ATP + H2O → carbamoyl phosphate + glutamate + 2 ADP + Pi. One ATP activates bicarbonate and a second phosphorylates carbamate; ammonia generated in the glutaminase domain is channeled internally. This cytosolic reaction is the committed, rate-limiting, and effectively irreversible step of mammalian pyrimidine de novo synthesis; it must not be confused with mitochondrial CPS-I of the urea cycle.
Showing all 1 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
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