What it does, reaction by reaction
Purine De Novo Synthesis (IMP synthesis through to AMP/GMP) Nucleotide Metabolism · Cytosol
PRPP → 5-phosphoribosylamine (5-PRA)
Converts PRPP into 5-Phosphoribosylamine
› Notes
Glutamine-PRPP amidotransferase catalyzes PRPP + glutamine + H2O → 5-PRA + glutamate + PPi. The enzyme uses an internal glutaminase domain to generate ammonia, which is channeled to the phosphoribosyl-transferase domain; PPi hydrolysis drives the reaction forward. This is the committed, rate-limiting, and effectively irreversible step of de novo purine synthesis.
Showing all 1 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
Purine De Novo Synthesis (IMP synthesis through to AMP/GMP)
PRPP
IMP, AMP, and GMP (feedback inhibition; combined nucleotide pools are most effective)
No established direct endocrine regulation; expression/flux increase with growth signals and proliferation.
Recent literature
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