Long-chain acyl-CoA synthetase (ACSL/thiokinase)
Cofactors used
What it does, reaction by reaction
Beta-Oxidation of Fatty Acids Lipid Metabolism · Mitochondrial matrix (peroxisome for VLCFA)
Fatty acid + CoA-SH + ATP → fatty acyl-CoA + AMP + PPi
Converts Fatty acid CoA-SH ATP into Fatty acyl-CoA AMP PPi
› Notes
A long-chain acyl-CoA synthetase (thiokinase) activates the fatty acid at the outer mitochondrial membrane/ER. ATP is converted to AMP and pyrophosphate; pyrophosphatase hydrolysis makes activation effectively irreversible and costs two ATP equivalents. This is necessary before transport or oxidation but is not the rate-limiting control point for long-chain oxidation.
Showing all 1 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
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