Dihydrolipoyl transacetylase (E2)
Cofactors used
What it does, reaction by reaction
Pyruvate Oxidation / Link Reaction (Pyruvate Dehydrogenase Complex) Carbohydrate Metabolism · Mitochondrial matrix
Acetyl-lipoamide + CoA-SH → acetyl-CoA + dihydrolipoamide
Converts Acetyl-lipoamide CoA-SH into Acetyl-CoA Dihydrolipoamide
› Notes
E2 transfers the acetyl group to coenzyme A (CoA-SH), creating acetyl-CoA. This is a transesterification reaction; CoA is derived from pantothenate (vitamin B5).
Pyruvate Dehydrogenase Complex (Link Reaction, connecting glycolysis to the TCA cycle) Energy Metabolism & Cellular Respiration · Mitochondrial matrix
Hydroxyethyl-TPP + oxidized lipoamide-E2 → acetyl-dihydrolipoamide-E2 + TPP
Converts Hydroxyethyl-TPP Oxidized lipoamide-E2 into Acetyl-dihydrolipoamide-E2 Thiamine pyrophosphate (TPP)
› Notes
Required cofactors: TPP and lipoic acid/lipoamide. The lipoamide “swinging arm” channels intermediates among active sites.
Acetyl-dihydrolipoamide-E2 + CoA-SH → acetyl-CoA + dihydrolipoamide-E2
Converts Acetyl-dihydrolipoamide-E2 CoA-SH into Acetyl-CoA Dihydrolipoamide-E2
› Notes
Required cofactor: CoA, whose reactive thiol forms the acetyl thioester. Acetyl-CoA formation is part of the overall irreversible, committed entry of pyruvate carbon into oxidative metabolism; acetyl-CoA cannot be converted back to pyruvate in humans.
Showing all 3 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
Europe PMC · fetched just now · sorted by publication date
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1
A biocompatible copper based therapeutic nanoplatform for CD44 specific tumor targeted therapy and potent immune reprogramming.
Fang Y, Tu M, Tu X, Zhang H, Li Y, Xu J. · 2026-06-13
unreviewed -
2
Suppression of LncRNA AC008406.3 sensitizes breast cancer cells to docetaxel via triggering cuproptosis.
Liu C, Liu S, Lu Z, Cao X, Tong X, Sun I, Hao J, Zhang L. · 2026-05-26
open access unreviewed -
3
Cuproptosis in spinal cord injury: emerging mechanisms and immunological relevance.
Li LH, Zhang JQ, Ying XH, Huang Y, Xu CC, Wang XX, He KL, Ma RJ. · 2026-03-25
open access unreviewed -
4
The role of copper and cuproptosis in digestive system cancers: novel therapeutic strategies and mechanistic insights.
Xu G, Yao N, Cheng R, Yang L, Han F, Qu J, Li W. · 2026-03-16
open access unreviewed -
5
The molecular mechanism of cuproptosis and research progress in pancreatic diseases.
Wang Q, Chen H, Lv Z, Zhang P, Li Y, Zhao C, Li S. · 2025-12-27
cited 2× open access unreviewed -
6
Pioneering next-generation bioactive materials for endodontics: Insights of mitochondrial biology.
Cheng S, Liu XY, Zhou L, Mao HQ, Wen YH, Meng X, Zhang L, Chen Z. · 2026-06-04
open access unreviewed -
7
Mitochondria-targeted peptide-engineered bimetallic nanozymes enable ferroptosis-sensitized cuproptosis for melanoma therapy.
Kong J, Cai M, Sun A, Zhao M, Li M, Wang X, Qiao J, Bao H, Zhao R, Xu B, Wang P, Tang Z. · 2026-07-22
unreviewed -
8
GSH-responsive self-assembled nanoplatform synergistically enhances cuproptosis through metabolic reprogramming and oxidative stress amplification.
Huang B, Zhang W, Wang Y, Luo X, Wu W, Shen R, Liu X, Zhang Z, Gao Y, Wu Y, Zeng F, Huang Y, Yu J, Wang S, Wu… · 2026-07-27
unreviewed -
9
Hyaluronic acid-camouflaged dendritic silica nanoparticles enable targeted cuproptosis and photothermal therapy for lung cancer.
Li X, Li C, Liu D, Wang Y. · 2026-06-02
unreviewed -
10
The role of cuproptosis in osteoporosis: Molecular mechanisms and cell-specific regulation in bone metabolic imbalance.
He J, Li F, Yuan H, Liu J, Peng Y, Wu H, Luo Y, Li F, Xu W, Song C, Hao P, Liu Z. · 2026-05-21
unreviewed
External claims. These come from an index outside this database and are not checked against it. Treat them as leads.