Dihydrolipoyl transacetylase (E2)
Cofactors used
What it does, reaction by reaction
Pyruvate Oxidation / Link Reaction (Pyruvate Dehydrogenase Complex) Carbohydrate Metabolism · Mitochondrial matrix
Acetyl-lipoamide + CoA-SH → acetyl-CoA + dihydrolipoamide
Converts Acetyl-lipoamide CoA-SH into Acetyl-CoA Dihydrolipoamide
› Notes
E2 transfers the acetyl group to coenzyme A (CoA-SH), creating acetyl-CoA. This is a transesterification reaction; CoA is derived from pantothenate (vitamin B5).
Pyruvate Dehydrogenase Complex (Link Reaction, connecting glycolysis to the TCA cycle) Energy Metabolism & Cellular Respiration · Mitochondrial matrix
Hydroxyethyl-TPP + oxidized lipoamide-E2 → acetyl-dihydrolipoamide-E2 + TPP
Converts Hydroxyethyl-TPP Oxidized lipoamide-E2 into Acetyl-dihydrolipoamide-E2 Thiamine pyrophosphate (TPP)
› Notes
Required cofactors: TPP and lipoic acid/lipoamide. The lipoamide “swinging arm” channels intermediates among active sites.
Acetyl-dihydrolipoamide-E2 + CoA-SH → acetyl-CoA + dihydrolipoamide-E2
Converts Acetyl-dihydrolipoamide-E2 CoA-SH into Acetyl-CoA Dihydrolipoamide-E2
› Notes
Required cofactor: CoA, whose reactive thiol forms the acetyl thioester. Acetyl-CoA formation is part of the overall irreversible, committed entry of pyruvate carbon into oxidative metabolism; acetyl-CoA cannot be converted back to pyruvate in humans.
Showing all 3 reactions.
What accelerates and inhibits it
Regulation is pathway-specific, so each context is listed separately
No regulation recorded for this enzyme in the source documents.
Recent literature
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